Multiple response optimization of heat shock process for separation of bovine serum albumin from plasma.

Autor: Gonzalez, U. A., Menendez, C., Saitua, H. A., Rigau, J.
Předmět:
Zdroj: Separation Science & Technology; 2017, Vol. 52 Issue 12, p1992-2001, 10p
Abstrakt: Bovine serum albumin produced by heat shock from bovine plasma has been researched for the effects of sodium caprylate concentration [Cap], temperature (T), and pH on yield (Yield%) and purity (BSA%). Response surface methodology and desirability function approaches were applied to optimize its process. The best compromise solution was found with BSA% = 95.0 and Yield% = 28.5 for a [Cap] = 2 mM,T= 67.9°C, and pH = 5. A Monte Carlo simulation showed that it is possible to obtain excellent values for each individual response. However, a techno-economic feasibility study must be carried out to determine which one is the best option. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index