Localization of Selenoproteins in the Perinuclear Structure of the Rat Kidney.

Autor: Kyriakopoulos, A., Hoppe, B., Graebert, A., Kühbacher, M., Weseloh, G., Behne, D.
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Zdroj: Instrumentation Science & Technology; Mar2004, Vol. 32 Issue 2, p221-232, 12p
Abstrakt: Investigations were performed on rats to obtain information on the distribution of selenoproteins in the microsomal fraction, especially in the perinuclear structure [endoplasmatic reticulum (ER) and Golgi apparatus] of the kidney. For the determination of the selenium levels instrumental neutron activation analysis (INAA) via 75Se was used. The membrane-bound selenium-containing proteins were detected by labeling of rats with 75Se-selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS-PAGE, or two-dimensional electrophoresis and autoradiography of 75Se. In this way, at least 17 membrane-bound selenium-containing proteins could be distinguished in the microsomal fraction of the kidney. Of those, six selenium-containing proteins with molecular masses of about 15, 16, 20, 23–25, 42–44, and 58–60 kDa were found in the ER fraction, which was isolated from the microsomes by differential centrifugation and further purification steps with detergents. The 75Se-labeled bands with molecular masses of 15 and 20 kDa and those in the range of 23–25, 42–44, and 58–60 kDa, could be attributed to known selenoproteins. A further 75Se-labeled 16 kDa protein, which had been detected in an earlier study, was characterized as a selenocysteine (Sec)-containing selenoprotein with an isoelectric point of 5.4. The localization of the different selenoproteins in the ER is of great interest with regard to the functions of these cellular organelles. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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