Study of conjunctival flora in anophthalmic patients: influence on the comfort of the socket.

Autor: Toribio, Alvaro, Marrodán, Teresa, Fernández-Natal, Isabel, Martínez-Blanco, Honorina, Rodríguez-Aparicio, Leandro, Ferrero, Miguel
Předmět:
Zdroj: Graefe's Archive of Clinical & Experimental Ophthalmology; Aug2017, Vol. 255 Issue 8, p1669-1679, 11p
Abstrakt: Purpose: To investigate the relationship between conjunctival flora and comfort of the socket in anophthalmic patients. Methods: A cross-sectional clinical study including 60 patients with unilateral anophthalmia who wear a prosthetic eye. From each patient three microbiological samples were taken from the lower conjunctival sac (healthy eye, pre-prosthesis, and retro-prosthesis space of socket). The 180 samples obtained were cultured. Samples from a randomized subgroup of 29 patients were measured by spectrophotometry at 540 nm after 48 h of growth, to determine their microbial density (MD). The grade of comfort of the socket (GCS) of each patient was established by a questionnaire. Epidemiological and clinical data of the anophthalmic socket and artificial eye care of each patient were also collected. Results: MD decreased in healthy eyes (0.213 ± 0.201, P = 0.004) compared with the pre-prosthesis (0.402 ± 0.323) and retro-prosthesis (0.438 ± 0.268) samples. Pre-prosthesis MD correlated with retro-prosthesis MD ( R = 0.401, P = 0.031) and healthy eye MD ( R = 0.482, P = 0.008), and it was also related to poor GCS ( P = 0.017). Aerobic Gram-negative bacteria in retro-prosthesis samples of patients with poor GCS was higher than in patients with good or fair GCS ( P = 0.008). In the same samples, coagulase-negative staphylococci proportion (excluding S. epidermidis) increased in patients with good GCS ( P = 0.030). Conclusions: Socket microflora is related to GCS. Increased pathogenic flora, especially Gram-negative bacteria, and high MD are related to discomfort, while coagulase-negative staphylococci (other than S. epidermidis) are associated with comfort. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: