Isolation and Identification of Sediment derived Actinomycetes through Molecular Characterization of 16s rRNA Technique.

Autor: Velayutham, G., Jenifer, S., Kumar, S. Senthil
Předmět:
Zdroj: Journal of Plant Science Research; Jan-Jun2017, Vol. 33 Issue 1, p57-61, 5p, 3 Charts, 1 Graph
Abstrakt: To investigate, isolate and identification, characterizations of Bioactive potential cytotoxic effect of actinomycetes collected from turmeric crop plantation in Erode, District. The soil samples were air dried under in vitro lab condition for 3 – 4 days. After air drying it has been washed two times with the help of distilled water to remove the unwanted dust particles from the soil sample. The soil samples was subjected into the basic microbiological techniques such as, serial dilution, pour plate and spread plate methods, that is to identify the specific growth of actinomycetes and its identified that it is present in the 10-3 and 10-4. Followed by the microbiological procedure, finally the samples were undergone for identifying the biochemical characterization and using various parameters of the biochemical test like Methyl red, Vogues-Proskauer, Nitrate Reduction were clearly performed for the identification. After the isolation procedure is completed the sample was subjected to the 16s rRNA sequencing analysis of selected actionomycetes. Isolated actinomycetes culture was identified by 16s rRNA sequencing and it was confirmed through the bioinformatics tools as BLAST. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index