Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).

Autor: Wu, Qinglin, Fenton, Benjamin A., Wojtaszek, Jessica L., Zhou, Pei
Předmět:
Zdroj: Chemical Communications; 8/7/2017, Vol. 53 Issue 61, p8541-8544, 4p
Abstrakt: CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index