| Autor: |
Héritier, Sébastien, Hélias ‐ Rodzewicz, Zofia, Lapillonne, Hélène, Terrones, Nathalie, Garrigou, Sonia, Normand, Corinne, Barkaoui, Mohamed ‐ Aziz, Miron, Jean, Plat, Geneviève, Aladjidi, Nathalie, Pagnier, Anne, Deville, Anne, Gillibert ‐ Yvert, Marion, Moshous, Despina, Lefèvre ‐ Utile, Alain, Lutun, Anne, Paillard, Catherine, Thomas, Caroline, Jeziorski, Eric, Nizard, Philippe |
| Předmět: |
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| Zdroj: |
British Journal of Haematology; Aug2017, Vol. 178 Issue 3, p457-467, 11p |
| Abstrakt: |
The BRAF V600E mutation is reported in half of patients with Langerhans cell histiocytosis ( LCH). This study investigated the detection of the BRAF V600E allele in circulating cell-free (ccf) DNA in a paediatric LCH cohort. Children with BRAFV600E-mutated LCH were investigated to detect ccf BRAF V600E at diagnosis ( n = 48) and during follow-up ( n = 17) using a picolitre-droplet digital PCR assay. At diagnosis, ccf BRAFV600E was positive in 15/15 (100%) patients with risk-organ positive multisystem ( RO+ MS) LCH, 5/12 (42%) of patients with RO− MS LCH and 3/21 (14%) patients with single-system ( SS) LCH ( P < 0·001, Fisher's exact test). The positive BRAFV600E load was higher for RO+ patients (mean, 2·90%; range, 0·04-11·4%) than for RO− patients (mean, 0·16%; range, 0·01-0·39) ( P = 0·003, Mann-Whitney U test). After first-line vinblastine-steroid induction therapy, 7/7 (100%) of the non-responders remained positive for ccf BRAFV600E compared to 2/4 (50%) of the partial-responders and 0/4 of the complete responders ( P = 0·002, Fisher's exact test). Six children treated with vemurafenib showed a clinical response that was associated with a decrease in the ccf BRAFV600E load at day 15. Thus, ccf BRAFV600E is a promising biomarker for monitoring the response to therapy for children with RO+ MS LCH or RO− LCH resistant to first-line chemotherapy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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