| Autor: |
Al-Zubaydi, Sundus Saad, Dabija-Wolter, Gabriela, Mohammed, Marwan Mansoor Ali, Bolstad, Anne Isine |
| Předmět: |
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| Zdroj: |
Journal of Oral Microbiology; 2017 Supplement, Vol. 9, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Background:Antibiotic resistance has become life-threatening. A combination of metronidazole and amoxicillin is commonly used as adjunct to periodontal therapy. The aim of this study was to utilize anin vitrobiofilm model to test the effect of replacing the broad-spectrum amoxicillin with penicillin V in the combination with metronidazole. Methods:Anin vitrobiofilm model, consisting ofAggregatibacter actinomycetemcomitans, Porphyromonas gingivalisandFusobacterium nucleatumwas developed. Biofilms were exposed to chlorhexidine digluconate (CHX) 0.2%, as positive control, and either to metronidazole/amoxicillin or metronidazole/penicillin V in two different concentrations. Statistical significance was achieved if p < 0.05 with paired t-test. Results:There was no growth of any bacterial species after 2h exposure to 0.2% CHX. Together, the two combinations of antibiotics were effective againstF. nucleatumandP. gingivalis, but less effective againstA. actinomycetemcomitans. No significant difference was found between the two combinations of antibiotics to combatA. actinomycetemcomitansneither in high (p = 0.95) nor in low concentration (p = 0.17). Conclusion:A combination of metronidazole with penicillin V had similar inhibiting effect as metronidazole combined with amoxicillin on anin vitrothree-species biofilm model. Use of a narrow spectrum antibiotic may be indicated in combination with metronidazole. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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