| Autor: |
KEPEKCI TEKKELI, S. E., GAZIOGLU, I., KIZILTAS, M. V. |
| Předmět: |
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| Zdroj: |
Acta Chromatographica; Mar2017, Vol. 29 Issue 1, p57-65, 9p |
| Abstrakt: |
A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min-1. The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 μg mL-1. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 μg mL-1, respectively. Intra-day and interday repeatabilities were less than 3.15%. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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