| Autor: |
Ayob, Farah Wahida, Simarani, Khanom, Zainal Abidin, Nurhayati, Mohamad, Jamaludin |
| Předmět: |
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| Zdroj: |
Microbial Biotechnology; Jul2017, Vol. 10 Issue 4, p926-932, 7p |
| Abstrakt: |
This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography-mass spectrometry ( LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography ( HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer ( MDA- MB 231) was treated with a purified vinblastine which was intracellulary produced by N. sphaerica. The purified vinblastine from extracted leaf of C. roseus was used as a standard comparison. A positive result with a value of half maximal inhibitory concentration ( IC50) of > 32 μg ml−1 was observed compared with standard ( IC50) of 350 μg ml−1 only. It showed that a vinblastine produced by N. sphaerica has a high cytotoxicity activity even though the concentration of vinblastine produced by this endophytic fungus was only 0.868 μg ml−1. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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