RCH51, a multiply antibiotic-resistant Acinetobacter baumannii ST103IP isolate, carries resistance genes in three plasmids, including a novel potentially conjugative plasmid carrying oxa235 in transposon Tn6252.

Autor:	Hamidian, Mohammad, Nigro, Steven J., Hartstein, Rebecca M., Hall, Ruth M.
Předmět:	ACINETOBACTER baumannii MOBILE genetic elements DRUG resistance PLASMID incompatibility MOLECULAR genetics ACINETOBACTER ACINETOBACTER infections ANTIBIOTICS DNA DRUG resistance in microorganisms GENES GENETICS GENOMES GENTAMICIN HYDROLASES MICROBIAL sensitivity tests TOBRAMYCIN GRAM-negative aerobic bacteria SEQUENCE analysis PHARMACODYNAMICS
Zdroj:	Journal of Antimicrobial Chemotherapy (JAC); Jul2017, Vol. 72 Issue 7, p1907-1910, 4p
Abstrakt:	Objectives: To determine the identity and context of genes conferring antibiotic resistance in a sporadic multiply antibiotic-resistant Acinetobacter baumannii recovered at Royal Children's Hospital, Brisbane.Methods: The antibiotic resistance phenotype for 23 antibiotics was determined using disc diffusion or MIC determination. The whole-genome sequence of RCH51 was determined using the Illumina HiSeq platform. Antibiotic resistance determinants were identified using ResFinder. Plasmids were recovered by transformation.Results: Isolate RCH51 belongs to the uncommon STs ST103 IP (7-3-2-1-7-1-4) and ST514 OX (1-52-29-28-18-114-7). It was found to be resistant to sulfamethoxazole, tetracycline, gentamicin, tobramycin and kanamycin and also exhibited reduced susceptibility to imipenem (MIC 2 mg/L) and meropenem (MIC 6 mg/L). RCH51 carries the oxa235 , sul2 , floR , aadB and tet39 resistance genes, all located on plasmids. The largest of the three plasmids, pRCH51-3, is 52 789 bp and carries oxa235 in the ISAba1-bounded transposon Tn 6252 , as well as sul2 and floR . pRCH51-3 represents a new A. baumannii plasmid family that is potentially conjugative as it contains several genes predicted to encode transfer functions. However, conjugation of pRCH51-3 was not detected. The aadB and tet39 resistance genes were each found in small plasmids identical to the known plasmids pRAY*-v1 and pRCH52-1, respectively.Conclusions: The resistance gene complement of RCH51 was found in three plasmids. pRCH51-3, which carries the oxa235 , sul2 and floR resistance genes, represents a new, potentially conjugative A. baumannii plasmid type. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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