CARBOHYDRATE METABOLISM IN AGARICUS BISPORUS (LANGE) IMBACH.: METABOLISM OF [14C] LABELLED SUGARS BY SPOROPHORES AND MYCELIUM.

Autor:	Hammond, J. B. W., Nichols, R.
Předmět:	CARBOHYDRATE metabolism CULTIVATED mushroom MYCELIUM GLUTAMATE decarboxylase ALANINE metabolism PENTOSE phosphate pathway PLANT physiology
Zdroj:	New Phytologist; Sep77, Vol. 79 Issue 2, p315-325, 11p
Abstrakt:	When growing sporophores and vegetative mycelium of Agaricus bisporus had been supplied with [14C] labelled hexoses for varying periods of time, the major compounds labelled in both forms were mannitol, trehalose, glutamate, alanine and an unidentified substance. Mannitol was strongly labelled after application of [14C] fructose but only weakly when [14C] glucose was supplied. The relative rates of oxidation by sporophores of labelled mannitol, trehalose, glucose and fructose were assessed by measuring 14CO2 production. Glucose and trehalose were the most rapidly oxidized substrates. Glucosephosphatase isomerase (E.C. 5.3.1.9) was extracted from sporophores and assayed. The conversion of glucose-6-phosphate to fructose-6-phosphate by the enzyme was competitively inhibited in vitro by 6-phosphogluconate. The control of mannitol synthesis and the functions of mannitol and trehalose are discussed. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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