| Autor: |
Grainger, David C., Belyaeva, Tamara A., Lee, David J., Hyde, Eva I., Busby, Stephen J.W. |
| Předmět: |
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| Zdroj: |
Molecular Microbiology; Mar2004, Vol. 51 Issue 5, p1311-1320, 10p |
| Abstrakt: |
We have investigated the role of the RNA polymerase α subunit during MelR-dependent activation of transcription at the Escherichia coli melAB promoter. To do this, we used a simplified melAB promoter derivative that is dependent on MelR binding at two 18 bp sites, located from position −34 to −51 and from position −54 to −71, upstream of the transcription start site. Results from experiments with hydroxyl radical footprinting, and with RNA polymerase, carrying α subunits that were tagged with a chemical nuclease, show that the C-terminal domains of the RNA polymerase α subunits are located near position −52 and near position −72 during transcription activation. We demonstrate that the C-terminal domain of the RNA polymerase α subunit is needed for open complex formation, and we describe two experiments showing that the RNA polymerase α subunit can interact with MelR. Finally, we used alanine scanning to identify determinants in the C-terminal domain of the RNA polymerase α subunit that are important for MelR-dependent activation of the melAB promoter. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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