| Autor: |
Lenstra, Johannes A., van Vliet, Anja, Arnberg, Annika C., van Hemert, Formijn J., Möller, Wim |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 3/17/86, Vol. 155 Issue 3, p475-483, 9p |
| Abstrakt: |
The elongation factor EF-1a is one of the most abundant proteins in eukaryotic cells, where it catalyzes the binding of aminoacyl-tRNA to ribosomes. The genes coding for this protein in the brine shrimp Artemia were analyzed by gene cloning, electron microscopy and chromosomal blot hybridization. There are only a few (about four) copies of one type of gene per haploid genome. These genes contain five exons divided over 104 base pairs. Local rearrangements give rise to a number of gene variants. Cross-hybridizations of Anemia cDNA probes with yeast and Drosophila DNA revealed two different yeast EF-1a, genes and one or two different Drosophila genes, respectively. Nucleotide sequencing revealed signals for synthesis and processing of EF-1a transcripts as well as the exact location of exons. One interruption in the coding sequence corresponds closely to a splice junction in the gene coding for the homologous chloroplast protein EF-Tu from Euglena grad/is, presumably of prokaryotic origin. The first exon in the chloroplast gene codes for the region of EF-Tu that is homologous to regions of the elongation factor EF-G and of the initiation factor IF2, respectively. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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