| Autor: |
Gall, Andrew, Ellervee, Aleksandr, Robert, Bruno, Freiberg, Arvi |
| Předmět: |
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| Zdroj: |
FEBS Letters; Feb2004, Vol. 560 Issue 1-3, p221, 5p |
| Abstrakt: |
The effect of application of high pressure on the carotenoid-containing bacterial reaction centre from Rhodobacter sphaeroides strain 2.4.1 was studied, and compared to recent experiments performed on its carotenoid-less counterpart, isolated from strain R26.1. Our results indicate that the cavity created by the absence of carotenoid contributes to localised differences in protein compressibility when using the intrinsic chromophores as molecular probes. Differential stability of the electronic transitions of the primary electron donor under high hydrostatic pressure is observed, dependent on the presence of the carotenoid cofactor. This suggests that the transition intensity loss is induced by a slight change of the primary electron donor structure, allowed by the void created by the absence of the carotenoid molecule. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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