| Autor: |
Gröger, Marko, Lange, Matthias, Rennert, Knut, Kaschowitz, Tobias, Plettenberg, Holger, Hoffmann, Martin, Mosig, Alexander S. |
| Předmět: |
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| Zdroj: |
Engineering in Life Sciences; May2017, Vol. 17 Issue 5, p585-593, 9p |
| Abstrakt: |
Near infrared spectroscopy is a rapid and nondestructive method for compositional analysis of biological material. The technology is widely used within bioreactors and possesses potential as a standardized method for quality control in miniaturized microfluidic cell culture systems. Here, we established a method for quantification of cell density and viability of adherent HepaRG cells cultured in a translucent, miniaturized cell culture biochip. The newly developed statistical models for interpretation of near infrared spectroscopy from biochips are the basis for a novel method of fast, continuous, and contact-free analysis of cell viability and real-time monitoring of cell growth. The technique thus paves the way for a robust and reliable high-throughput analysis of biochip-embedded cell cultures. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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