| Autor: |
Dantas, Raquel Cristina Cavalcanti, Silva, Rebecca Tavares e, Ferreira, Melina Lorraine, Gonçalves, Iara Rossi, Araújo, Bruna Fuga, Campos, Paola Amaral de, Royer, Sabrina, Batistão, Deivid William da Fonseca, Gontijo-Filho, Paulo Pinto, Ribas, Rosineide Marques |
| Předmět: |
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| Zdroj: |
PLoS ONE; 5/8/2017, Vol. 12 Issue 5, p1-14, 14p |
| Abstrakt: |
The bacterial factors associated with bacteremia by multidrug-resistant and extensively drug-resistant P. aeruginosa, including overexpression of efflux pumps, AmpC overproduction, and loss/alteration of the OprD porin in isolates that are non-Metallo-β-Lactamase producing were analyzed in a retrospective study. Molecular analyses included strain typing by Pulsed Field Gel Electrophoresis and identification of key genes via qualitative and quantitative PCR-based assays. Previous use of carbapenems and tracheostomy was independently associated with the development of bacteremia by extensively drug-resistant and multidrug-resistant strains of P. aeruginosa. A high consumption of antimicrobials was observed, and 75.0% of the isolates contained amplicons with the blaSPM-1 and blaVIM genes. Of the 47 non-Metallo-β-Lactamase isolates, none had another type of carbapenemase. However, the isolates exhibited high rates of hyperproduction of AmpC, loss of the OprD porin (71.4%) and the presence of MexABOprM (57.1%) and MexXY (64.3%). This study suggests that in non-Metallo-β-Lactamase isolates, the association of intrinsic resistance mechanisms could contributes to the expression of multidrug-resistant/extensively drug-resistant phenotypes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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