| Autor: |
Bulloch, Travis M., Argyle, T. Charles, Parson, Madeline Gleave, Monson, Christopher F. |
| Předmět: |
|
| Zdroj: |
Journal of the Utah Academy of Sciences, Arts & Letters; 2016, Vol. 93, p269-281, 13p |
| Abstrakt: |
Cell membranes, which are essential to living cells, consist of a lipid bilayer with membrane proteins and other membrane components integrated into the membrane. The cell membrane separates the fluid inside the cell (the cytoplasm) from the fluid surrounding the cell. The arrangement of components within the cell membrane is also extremely important, particularly in cell communications. Supported lipid bilayers consist of a lipid bilayer a few nanometers above a solid support (often glass) and are a convenient platform with which to study membrane patterning. Previous work with supported lipid bilayers has explored several methods by which various grid-based patterns can be formed in the cell membrane in an attempt to interact with cellular communication at a molecular level. The previously explored gridbased patterns do not provide a way to generate the type of circular pattern that would be required for cell synapses. We generated circular patterns using supported lipid bilayers containing a small amount of fluorescein-labeled lipids. We then introduced superparamagnetic beads coated with anti-fluorescein antibodies, causing the aggregation of fluorescein-labeled lipids into a circular pattern under each bead. The beads were removed from the surface with a magnet, and pattern formation was confirmed by fluorescence microscopy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
