| Autor: |
Kurekci, Cemil, Pehlivanlar Önen, Sevda |
| Zdroj: |
Journal of Food Measurement & Characterization; Jun2017, Vol. 11 Issue 2, p586-591, 6p |
| Abstrakt: |
The objective of the current study was to investigate mutations in the gyrA and 23S rRNA gene fragments the high level ciprofloxacin resistant (HL-Cip) Campylobacter isolates ( C. jejuni n = 6 and and C. coli n = 9) obtained from chicken meat samples, in Turkey. PCR-based restriction fragment length polymorphism (RFLP) was used to characterize these isolates. In addition, the presence of several virulence traits among these isolates was also examined. Of the 15 HL-Cip Campylobacter strains, there were eight unique RFLP banding patterns. All HL-Cip Campylobacter strains had mutations in codon 86 (Thr-86 to Ile) in the gyrA gene. Four C. jejuni isolates had missense mutation of Asp-203 to Ser, whereas one C. jejuni strain also presented a change at Ala-40 to Ser. A2075G substitution in the 23S rRNA gene was identified in five isolates ( C. coli n = 3 and C. jejuni n = 2), whereas none of the isolates had A2074G substitution. The tetO gene conferring resistance to tetracycline were observed among five Campylobacter isolates. Of Campylobacter strains, 13 (86.6 %) were found to be positive for one or more virulence factors, the cdt genes being the most detected. The results of the current study extends the current knowledge about molecular mechanisms for erythromycin and ciprofloxacin resistance as well as virulence traits by investigation of HL-Cip Campylobacter isolates from chicken meat sold in Turkey. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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