In Vitro Evaluations and In Vivo Toxicity and Efficacy Studies of MFM501 against MRSA.

Autor:	Johari, Saiful Azmi, Mohtar, Mastura, Syed Mohamad, Sharifah Aminah, Mohammat, Mohd Fazli, Sahdan, Rohana, Mohamed, Azman, Mohamad Ridhwan, Mohamad Jemain
Předmět:	PERITONITIS STAPHYLOCOCCAL disease prevention ALKALOIDS ANIMAL experimentation ANTIBIOTICS CELL membranes DOSE-effect relationship in pharmacology DRUG toxicity HOST-bacteria relationships MICE MICROBIAL sensitivity tests MICROSCOPY ORAL drug administration TIME METHICILLIN-resistant staphylococcus aureus DESCRIPTIVE statistics IN vitro studies IN vivo studies PHARMACODYNAMICS PREVENTION
Zdroj:	BioMed Research International; 4/27/2017, Vol. 2017, p1-10, 10p
Abstrakt:	Previously we have discovered a synthetically derived pyrrolidone alkaloid, MFM501, exhibiting good inhibitory activity against 53 MRSA and MSSA isolates with low cytotoxicity against three normal cell-lines with IC50 values at >625 µg/ml. Time-kill assay, scanning electron microscopy (SEM) analysis, in vivo oral acute toxicity test, and mice peritonitis model were carried out in this study. In the time-kill study, MFM501 showed a less than 3 log10 decrease in bacterial colony concentration value (CFU/ml) which represented a bacteriostatic action while displaying a time-dependent inhibitory mechanism. Following that, SEM analysis suggested that MFM501 may exert its inhibitory activity via cytoplasmic membrane disruption. Moreover, MFM501 showed no toxicity effect on treated mice at an estimated median acute lethal dose (LD50) value of more than 300 mg/kg and less than 2000 mg/kg. For the efficacy test, a mean effective dose (ED50) of 87.16 mg/kg was obtained via a single dose oral administration. Our data demonstrated that MFM501 has the potential to be developed further as a new, safe, and effective oral-delivered antibacterial agent against MRSA isolates. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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