Development of a Gene Expression Assay for the Diagnosis of Mycobacterium bovis Infection in African Lions ( Panthera leo).

Autor: Olivier, T. T., Viljoen, I. M., Hofmeyr, J., Hausler, G. A., Goosen, W. J., Tordiffe, A. S. W., Buss, P., Loxton, A. G., Warren, R. M., Miller, M. A., Helden, P. D., Parsons, S. D. C.
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Zdroj: Transboundary & Emerging Diseases; Jun2017, Vol. 64 Issue 3, p774-781, 8p
Abstrakt: Mycobacterium bovis infection, the cause of bovine tuberculosis ( BTB), is endemic in wildlife in the Kruger National Park ( KNP), South Africa. In lions, a high infection prevalence and BTB mortalities have been documented in the KNP; however, the ecological consequences of this disease are currently unknown. Sensitive assays for the detection of this infection in this species are therefore required. Blood from M. bovis-exposed, M. bovis-unexposed, M. tuberculosis-exposed and M. bovis-infected lions was incubated in Quanti FERON®- TB Gold ( QFT) tubes containing either saline or ESAT-6/ CFP-10 peptides. Using qPCR, selected reference genes were evaluated for expression stability in these samples and selected target genes were evaluated as markers of antigen-dependent immune activation. The abundance of monokine induced by gamma interferon ( MIG / CXCL9) mRNA, measured in relation to that of YWHAZ, was used as a marker of ESAT-6/ CFP-10 sensitization. The gene expression assay results were compared between lion groups, and lenient and stringent diagnostic cut-off values were calculated. This CXCL9 gene expression assay combines a highly specific stimulation platform with a sensitive diagnostic marker that allows for discrimination between M. bovis-infected and M. bovis-uninfected lions. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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