Binding of aflatoxin B 1 to cell wall components of Lactobacillus rhamnosus strain GG.

Autor: Lahtinen, S.J., Haskard, C.A., Ouwehand, A.C., Salminen, S.J., Ahokas, J.T.
Předmět:
Zdroj: Food Additives & Contaminants; Feb2004, Vol. 21 Issue 2, p158-164, 7p
Abstrakt: The surface of Lactobacillus rhamnosus strain GG (LGG) has previously been shown to bind aflatoxin B 1 (AFB 1 ) effectively, it being a food-borne carcinogen produced by certain species of Aspergillus fungi. To establish which components of the cell envelope are involved in the AFB 1 binding process, exopolysaccharides and a cell wall isolate containing peptidoglycan were extracted from LGG and its AFB 1 binding properties were tested. LGG was also subjected to various enzymatic and chemical treatments and their effects on the binding of AFB 1 by LGG were examined. No evidence was found for exopolysaccharides, cell wall proteins, Ca 2+ or Mg 2+ being involved in AFB 1 binding. The AFB 1 binding activity of the cell wall isolate indicates that AFB 1 binds to the cell wall peptidoglycan of LGG or compounds tightly associated with the peptidoglycan. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index