Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method.

Autor:	Zeman, David, Kušnierová, Pavlína, Bojková, Jana, Všianský, František, Zapletalová, Olga
Předmět:	IMMUNOGLOBULIN G CEREBROSPINAL fluid ENZYME-linked immunosorbent assay IMMUNOGLOBULINS IMMUNOBLOTTING
Zdroj:	Journal of Immunoassay & Immunochemistry; 2017, Vol. 38 Issue 2, p165-177, 13p
Abstrakt:	IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited. [ABSTRACT FROM PUBLISHER]
Databáze:	Complementary Index
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