Ilex paraguariensis crude extract acts on protection and reversion from damage induced by t-butyl hydroperoxide in human erythrocytes: a comparative study with isolated caffeic and/or chlorogenic acids.

Autor: Portela, José Luiz, Soares, Deividi, Rosa, Hemerson, Roos, Daniel Henrique, Pinton, Simone, Ávila, Daiana Silva, Puntel, Robson L
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Zdroj: Journal of the Science of Food & Agriculture; May2017, Vol. 97 Issue 7, p2007-2014, 8p
Abstrakt: BACKGROUND Studies comparing the effects of phytochemicals under different regimens of exposure are necessary to give a better indication about their mechanism(s) of protection. Hence, in the present study, we investigated the preventive (pre-incubation), protective (co-incubation) and/or remediative (post-incubation) activity of chlorogenic acid and caffeic acids, in comparison with Ilex paraguariensis crude extract, against t-butyl hydroperoxide (t- BHP)-induced damage to human erythrocytes. RESULTS We found that both caffeic and chlorogenic acids were able to prevent and revert the hemolysis associated with t- BHP exposure. By contrast, isolated compounds (alone or in combination) presented no effect on basal and/or t- BHP-induced non-protein thiol ( NPSH) oxidation or production of thiobarbituric acid reactive substances ( TBBARS). In turn, I. paraguariensis extract was effective to prevent, protect and revert the hemolysis associated with t- BHP exposure. Moreover, I. paraguariensis significantly protects and reverts t- BHP-induced NPSH oxidation and TBARS production. CONCLUSIONS We have found that I. paraguariensis extract acts better with respect to the protection and reversion of t- BHP-associated changes, whereas isolated compounds are more active in preventing and reverting t- BHP pro-hemolytic action. Moreover, our data suggest that the pro-hemolytic activity of t- BHP may occur via mechanism(s) other(s) than lipid peroxidation and/or NPSH oxidation. © 2016 Society of Chemical Industry [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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