| Autor: |
Wilken, Jason A., Matsumoto, Kikuko, Laughlin, Lisa S., Lasley, Bill L., Bedows, Elliott |
| Předmět: |
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| Zdroj: |
American Journal of Primatology; Feb2002, Vol. 56 Issue 2, p89-97, 9p |
| Abstrakt: |
We have designed novel DNA primers that allow us to detect the expression of the subunits of chorionic gonadotropin (CG) from a variety of species of the order Primates. Using these primers, reverse transcriptase-polymerase chain reaction (RT-PCR), and standard cloning techniques, we detected the expression of a single gene for the common glycoprotein hormone (GPH) α-subunit and at least two genes for the CG β-subunit in trophoblasts of Macaca fascicularis (cynomolgous macaque (cm)) at gestational day (GD) = 26 (± 2d). No cmCG expression was detected at GD = 35–40. When sequences of cmGPH-α and cmCG-β genes were compared to the corresponding genes of other primates, we found that the α-subunit of M. fascicularis was highly conserved compared to other primate species. However, cmCG β-subunits appeared to be less conserved, residing between those of human CG-β and baboon CG-β when analyzed phylogenetically. Of particular interest was a three amino acid stretch in one of the expressed cmCG-β genes that is distinct from all other primates studied. Our findings imply that not only does the expression of multiple CG β-subunit genes appear to be common to Old World monkeys, but that the presented methodology will greatly facilitate our ability to understand primate evolution. Am. J. Primatol. 56:89–97, 2002. © 2002 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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