| Autor: |
Pope, Charles E., Dresser, Betsy L., Chin, Neeoo W., Liu, James H., Loskutoff, Naida M., Behnke, Erica J., Brown, Corrine, Mcrae, Molly A., Sinoway, Charles E., Campbell, Mark K., Cameron, Kenneth N., Owens, O'Dell M., Johnson, Chad A., Evans, Ronald R., Cedars, Marcelle I. |
| Předmět: |
|
| Zdroj: |
American Journal of Primatology; Mar97, Vol. 41 Issue 3, p247-260, 14p |
| Abstrakt: |
A 21-year-old multiparous female exhibiting 31–41 day menstrual cycles was given hFSH (225 IU/day, Metrodin 75, from cycle day 3 through 9 (menses = day 1) and hCG (10,000 IU, Profasi, on day 10 to stimulate follicular development. At 35 h after hCG, under isoflurane (AErrane) anesthesia, follicles were aspirated by controlled suction under transvaginal ultrasound guidance. Metaphase II oocyctes (n = 11) were placed in modified human tubal fluid (mHTF, 100 μl) medium under oil at 37°C in humidified 5% CO[sub 2] . Frozen semen, collected by voluntary ejaculation, was thawed (70°C H[sub 2] O bath, 6 sec), diluted slowly, centrifuged, and resuspended in mHTF, and 160,000 motile spermatozoa/ml were added at 6 h after oocyte recovery. At 21 h postinsemination (p.i.) eight oocytes were at the two-cell stage, five were cryopreserved, and three were cultured to the six- to eight-cell stage in mHTF with granulosa cells before transcervical uterine transfer at 47 h p.i. using a Teflon catheter. Micronized progesterone (400 mg/d) was orally administered for 10 weeks posttransfer (p.t.). Ultrasound examination revealed a single fetus at 15 weeks p.t., and unassisted delivery of a live 1.37 kg female infant occurred at 29 weeks. Am. J. Primatol. 41:247–260, 1997. © 1997 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text