Cross-linking between 16S ribosomal RNA and protein S4 in Escherichia coli ribosomal 30S subunits effected by treatment with bisulfite/hydrazine and bromopyruvate.

Autor: Nitta, Noriko, Kuge, Osamu, Yui, Seiko, Negishi, Kazuo, Hayatsu, Hikoya
Předmět:
Zdroj: European Journal of Biochemistry; 6/2/86, Vol. 157 Issue 2, p427-432, 6p
Abstrakt: Reports on the modification of cytosine in nucleic acids by treatment with a mixture of bisulfite and hydrazine. Specificity of the reaction for single-stranded regions of nucleic acids with the product being N[sup 4]-aminocytosine. Use of bromopyruvate for the alkylation of protein SH groups; Labeling of the protein portion and digestion of the RNA portion with nucleases.
Databáze: Complementary Index