| Autor: |
Martínez, José, Machado, Valéria, Caballero-Gaitán, Susana, Santos, Maria, Alencar, Rodrigo, Escobar L., Maria, Hrbek, Tomas, Farias, Izeni |
| Zdroj: |
Conservation Genetics Resources; Mar2017, Vol. 9 Issue 1, p29-33, 5p |
| Abstrakt: |
We developed nuclear SNPs for the heavily overfished tambaqui/cachama negra Colossoma macropomum using next-generation sequencing-based genotyping. We created a reduced-representation library for 30 individuals from Orinoco and Amazon basins following the ddRAD methodology for sequencing on the IonTorrent PGM. We analyzed the sequence data using the software Stacks. We detected 22,476 tags characterized by 7,181 polymorphic sites, recovering 177 loci (268 SNPs) shared between both basins. A significant difference in the allelic frequencies was observed between basins for a subset of these SNPs. Thirty-nine SNPs could be useful for conservation/population genetics using traditional PCR-based genotyping methods. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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