| Abstrakt: |
The purpose of the research was to study the composition and antimicrobial, antioxidant activities of endophytes in tuberous root of Solanum tuberosum. Endophytes were isolated by using an exterior sterilisation method, classified and identified by morphological and molecular characters. Antimicrobial activities of endophytes were measured by using paper disc diffusion method. The in vitro scavenging activities on hydroxy radical and DPPH were investigated. 7 strains Gibberella moniliformis, 1 strain Diaporthe, 1 strain Alternaria nees, 1 strain uncultured and 1 strain Glomerularia sp. were isolated. Fermented liquid extracts of ethyl acetate (ACE) of 5 strains, n-butyl alcohol (BUE) of 3 strains had powerful widely antibacterial spectrum against all 6 indicator bacteria (Staphylococcus aureus, Bacillus licheniformis, Streptococcus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) with the inhibition zone diameter (IZD) over than 15 mm at the concentration of 5 mg/ml, MIC values ranges measured are 0.3125-2.500 mg/ml. ACE of 4 strains and BUE of 4 strains shows notable hydroxy radical scavenging abilities, with scavenging rates of 31.15-46.68% at the concentration of 0.8 mg/ml. Endophytic strains with antimicrobial and antioxidant activities were obtained to explore the application of endophytic resources from S. tuberosum. [ABSTRACT FROM AUTHOR] |
