| Autor: |
Battogtokh, Gantumur, Gotov, Oyuntuya, Ko, Young |
| Zdroj: |
Archives of Pharmacal Research; Mar2017, Vol. 40 Issue 3, p356-363, 8p |
| Abstrakt: |
Gold nanoparticles are promising materials for many applications that include imaging, drug delivery, and photothermal therapy. However, AuNPs can be unstable and/or toxic. We purposed to improve the stability and reduce toxicity of gold nanorods (AuNR) upon coating with biocompatible polymer, chitosan-ceramide (CS-CE), without replacing the original layer, CTAB. CS-CE-coated AuNR was prepared by simple mixing for 24 h and purified by centrifugation. The coating was confirmed by UV-Vis absorption analysis and surface charge and size measurement. We prepared nanorods with CS or CS-CE coating at two different concentrations (5 and 10% AuNR), the resulting in larger nanorods with a more positive surface-charge than that of AuNR. We investigated the UV-absorption and protein adsorption of the polymer coated nanorods. Based on the protein adsorption, AuNR-CS-CE was found to be more stable under physiological conditions than AuNR-CS. The cell internalization assay revealed that Hela cells internalized higher amounts of AuNR-CS-CE than that of AuNR-CS. Cytotoxicity study revealed that AuNR-CS-CE has lower toxicity than AuNR against HeLa cells. The CS-CE coating improved the stability of AuNR under physiological conditions via the hydrophobic interactions between the AuNR lipid surface and the ceramide anchor in the CS backbone as well as. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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