| Autor: |
ÇEVİK, Özge, ADIGÜZEL, Zelal, BAYKAL, Ahmet Tarık, ŞENER, Azize |
| Předmět: |
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| Zdroj: |
Turkish Journal of Biology; 2017, Vol. 41 Issue 1, p31-40, 10p |
| Abstrakt: |
Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS specific inhibitor, such as at 1400 W for 1 h at 37 °C in the presence of 5 μM ADP. According to the results, the levels of PS exposure and P-selectin binding were significantly higher in TNF-α + ADP platelets, which decreased with the addition of 1400 W. A significant induction in cGMP, Cyt-c, and Ca2+ levels was observed in platelets treated with TNF-α + ADP. On the other hand, the upregulation of the apoptotic gene caspase-3 and iNOS expression levels in TNF-α-treated and ADP-activated platelets was significantly reversed with the addition of 1400 W. These data demonstrate that TNF-α promotes iNOS expression through caspase-3 stimulation in human platelets, and its concomitance leads to the triggering of apoptosis-mediated inflammation upon platelet activation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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