| Autor: |
Sofijan, Hadi, Shun, Tan Joo, Abbasiliasi, Sahar, Mustafa, Shuhaimi, Puspaningsih, Nyoman Tri, Kadkhodaei, Saeid, Ariff, Arbakariya B. |
| Předmět: |
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| Zdroj: |
Separation Science & Technology; 2017, Vol. 52 Issue 5, p834-842, 9p |
| Abstrakt: |
A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinantBacillus megateriumMS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VRof 2.3, crude loading (CL) of 30% (w/w) at pH 6. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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