Construction of stable BHK-21 cells coexpressing human secretory glycoproteins and human Gal(β1-4)GlcNAc-R α2,6-sialyltransferase α2,2-linked NeuAc is preferentially attached to Gal(β1-4)GlcNAc(β1-2)Man(α1-3)-branch of diantennary oligosaccharides from secreted recombinant β-trace protein

Autor: Grabenhorst, Eckart, Hoffmann, Andrea, Nimtz, Manfred, Zettlmeissl, Gerd, Conradt, Harald S.
Předmět:
Zdroj: European Journal of Biochemistry; 9/15/95, Vol. 232 Issue 3, p718-725, 8p
Abstrakt: Reports that the human beta-trace protein has been cloned and has been expressed for the first time in a mammalian host cell line. Construction of stable BHK-21 cell lines exhibiting altered terminal sialylation properties; Support for previously reported in vitro branch specificity of the bovine colostrum Gal(beta1-4)GlcNAc-R alpha2,6-sialyltransferase gene activity; Suitability for the production of human-type glycosylated secretory recombinant polypeptides.
Databáze: Complementary Index