| Autor: |
Éltes, Tímea, Kirizs, Tekla, Nusser, Zoltan, Holderith, Noemi |
| Předmět: |
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| Zdroj: |
Journal of Neuroscience; 2/15/2017, Vol. 37 Issue 7, p1910-1924, 15p |
| Abstrakt: |
Target cell type-dependent differences in presynaptic release probability (Pr) and short-term plasticity are intriguing features of cortical microcircuits that increase the computational power of neuronal networks. Here we tested the hypothesis that different voltage-gated Ca2+ channel densities in presynaptic active zones (AZs) underlie different Pr. Two-photon Ca2+ imaging, triple immunofluorescent labeling and three-dimensional electron microscopic (EM) reconstruction of rat CA3 pyramidal cell axon terminals revealed approximately ~1.7 -- 1.9-times higher Ca2+ inflow per AZ area in high Pr boutons synapsing onto parvalbumin positive interneurons than in low Pr boutons synapsing onto mGluR1α-positive interneurons. EM replica immunogold labeling, however, demonstrated only 1.15-times larger Cav2.1 and Cav2.2 subunit densities in high Pr AZs. Our results indicate target cell type-specific modulation of voltage-gated Ca2+ channel function or different subunit composition as possible mechanisms underlying the functional differences. In addition, high Pr synapses are also characterized by a higher density of docked vesicles, suggesting that a concerted action of these mechanisms underlies the functional differences. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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