| Autor: |
Chiang, Chih-Ming, Chen, Chiu-Chen, Chen, Shi-Peng, Lin, Kuan-Hung, Chen, Li-Ru, Su, Yu-Huei, Yen, His-Cheng |
| Předmět: |
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| Zdroj: |
Journal of Plant Research; Mar2017, Vol. 130 Issue 2, p373-386, 14p |
| Abstrakt: |
Previously, we found that the flood resistance of eggplant ( Solanum melongena) and sponge gourd ( Luffa cylindrica) enhanced ascorbate peroxidase (APX) activity under flooding, and consequently, both the SmAPX and LcAPX genes were cloned. In this study, the SmAPX and LcAPX genes were transferred under a ubiquitin promoter to Arabidopsis (At) via Agrobacterium tumefaciens. The expression and amount of APX and APX activities of the SmAPX and LcAPX transgenic lines were significantly higher than those of non-transgenic (NT) plants under a waterlogged condition. Furthermore, the SmAPX, LcAPX, At-sucrose synthases ( SUS)-1, phosphoenolpyruvate carboxylase ( PEPC), and lactate dehydrogenase ( LDH) genes were overexpressed in all transgenic Arabidopsis lines after flooding treatment. Compared to NT plants, the malondialdehyde (MDA) contents and HO accumulation were significantly lower, but germination rates were significantly higher in all transgenic lines with higher APX activity, indicating that the overexpression of SmAPX and LcAPX in Arabidopsis could enhance flood tolerance by eliminating HO. Moreover, Arabidopsis seedlings overexpressing SmAPX and LcAPX also displayed greater resistance to flooding and less oxidative injury than NT plants subjected to flooding condition. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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