Permanganate-assisted removal of PCR inhibitors during the DNA Chelex extraction from stained denim samples.

Autor:	Pîrlea, Sorina, Puiu, Mihaela, Răducan, Adina, Oancea, Dumitru
Předmět:	PERMANGANATES POLYMERASE chain reaction NUCLEIC acid isolation methods DENIM EPITHELIAL cells
Zdroj:	International Journal of Legal Medicine; Mar2017, Vol. 131 Issue 2, p323-331, 9p
Abstrakt:	In this study, it was demonstrated that the DNA Chelex extraction combined with the permanganate assisted-oxidation is highly efficient in removing the PCR inhibitors often found in clothing materials, such as phthalocyanine. The extraction assays were conducted in saliva, blood and epithelial cells samples mixed with three oxidation-resistant dye copper(II) α-phthalocyanine, copper(II) β-phthalocyanine and tetrasulfonated copper(II) β-phthalocyanine. After DNA amplification, all samples were able to provide full DNA profiles. The permanganate/Chelex system was tested further on denim-stained samples and displayed the same ability to remove the PCR inhibitors from the commercial textile materials. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink