| Autor: |
Dib, Ikram, Fauconnier, Marie-Laure, Sindic, Marianne, Belmekki, Fatima, Assaidi, Asmae, Berrabah, Mohamed, Mekhfi, Hassane, Aziz, Mohammed, Legssyer, Abdelkhaleq, Bnouham, Mohamed, Ziyyat, Abderrahim |
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| Zdroj: |
BMC Complementary & Alternative Medicine; 1/31/2017, Vol. 17, p1-15, 15p, 2 Diagrams, 7 Graphs |
| Abstrakt: |
Background: Artemisia campestris L. (Asteraceae) is a medicinal herb traditionally used to treat hypertension and many other diseases. Hence, this study is aimed to analyze the essential oil of A. campestris L (AcEO) and to investigate the antiplatelet, antioxidant effects and the mechanisms of its vasorelaxant effect. Methods: The chemical composition of AcEO was elucidated using GC/MS analysis. Then, the antioxidant effect was tested on DPPH radical scavenging and on the prevention of β-carotene bleaching. The antiplatelet effect was performed on the presence of the platelet agonists: thrombin and ADP. The mechanism of action of the vasorelaxant effect was studied by using the cellular blockers specified to explore the involvement of NO/GC pathway and in the presence of calcium channels blockers and potassium channels blockers. Results: AcEO is predominated by the volatiles: spathulenol, β-eudesmol and p-cymene. The maximal antioxidant effect was obtained with the dose 2 mg/ml of AcEO. The dose 1 mg/ml of AcEO showed a maximum antiplatelet effect of, respectively 49.73% ± 9.54 and 48.20% ± 8.49 on thrombin and ADP. The vasorelaxation seems not to be mediated via NOS/GC pathway neither via the potassium channels. However, pretreatment with calcium channels blockers attenuated this effect, suggesting that the vasorelaxation is mediated via inhibition of L-type Ca2+ channels and the activation of SERCA pumps of reticulum plasma. Conclusion: This study confirms the antioxidant, antiplatelet and vasorelaxant effects of A.campestris L essential oil. However, the antihypertensive use of this oil should be further confirmed by the chemical fractionation and subsequent bio-guided assays. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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