| Autor: |
Petrova, L., Yordanova, A., Bogoeva, V. |
| Předmět: |
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| Zdroj: |
Genetics & Plant Physiology; 2016, Vol. 6 Issue 3/4, p135-141, 7p |
| Abstrakt: |
Adenine binding site has been identified in the lectin Concanavalin A (Con A), isolated from the legume plant Canavalia ensiformis. We found that, this protein bound with high affinity adenine with kD=0.38±0.08 μM. Emission spectra showed a significant decrease of the intrinsic tryptophan fluorescence, due to the interaction. The hyperbolic shape of the titration curve indicated presence of an "adenine" binding site. Additionally, enhancement of the extrinsic ANS fluorescence and sigmoidal shape of the titration curve revealed existence of hydrophobic sites within the protein molecule. The "adenine" binding site is suggested to have putative physiological role similar to many plant lectins. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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