Intrinsic blinking of red fluorescent proteins for super-resolution microscopy.

Autor: Klementieva, Natalia V., Pavlikov, Anton I., Moiseev, Alexander A., Bozhanova, Nina G., Mishina, Natalie M., Lukyanov, Sergey A., Zagaynova, Elena V., Lukyanov, Konstantin A., Mishin, Alexander S.
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Zdroj: Chemical Communications; 1/26/2017, Vol. 53 Issue 5, p949-951, 3p
Abstrakt: Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index