| Autor: |
Li, Yanshen, Zhang, Jing, Mao, Xin, Wu, Yongtao, Liu, Gongzhen, Song, Liting, Li, Yonggang, Yang, Jianrong, You, Yanli, Cao, Xuelin |
| Předmět: |
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| Zdroj: |
Journal of the Science of Food & Agriculture; Feb2017, Vol. 97 Issue 3, p818-822, 5p |
| Abstrakt: |
BACKGROUND T-2 toxin is a widely distributed mycotoxin in cereals. HT-2 toxin is the major metabolite, which is also a contaminant in cereals. T-2 toxin and HT-2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required. RESULTS In this study a high-sensitivity chemiluminescent enzyme-linked immunoassay ( CL-ELISA) of T-2 toxin and HT-2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC50 as low as 33.28 ng mL−1 and 27.27 ng mL−1 for T-2 and HT-2, respectively. In addition, this method had no cross-reaction with other structurally related mycotoxins. CONCLUSION These results indicated that the developed CL-ELISA could be applied for the detection of T-2 toxin and HT-2 toxin in actual samples without complicated steps. © 2016 Society of Chemical Industry [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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