| Autor: |
Andican, Gülnur, Geligen, Remise, Civelek, Sabiha, Seven, Arzu, Seymen, Oktay, Altug&tiled;, Tuncay, Yig&tiled;it, Günnur, Burçak, Gülden |
| Předmět: |
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| Zdroj: |
Journal of Toxicology & Environmental Health: Part A; 2004, Vol. 67 Issue 5, p413-420, 8p |
| Abstrakt: |
The effects of hyperthyroidism on oxidative DNA damage in liver tissue and modification by vitamin C supplementation were investigated in rats. Animals were rendered hyperthyroid by administration of L -thyroxine (0.4 mg/100 g food) for 25 d. In the plasma samples, T 3 , T 4 , and thyroid-stimulating hormone (TSH) were measured by radioimmunoassay and ascorbate spectrophotometrically. Oxidative damage to hepatic nuclear DNA was determined by measuring deoxy-guanosine (dG) and 8-oxodG by high-performance liquid chromatography with diode array detector electrochemical detection (HPLC-DAD-ECD). In hyperthyroidism, 8-oxodG/(10 5 dG) levels were significantly higher and plasma vitamin C levels lower than in control rats. The results of this experimental study show that oxidative damage to hepatic nuclear DNA increases in the hyperthyroid state and that vitamin C was not effective in preventing this damage. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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