| Autor: |
More, Tushar, Bagadi, Muralidhararao, RoyChoudhury, Sourav, Dutta, Mainak, Uppal, Annu, Mane, Anupama, Santra, Manas, Chaudhury, Koel, Rapole, Srikanth |
| Předmět: |
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| Zdroj: |
Metabolomics; Jan2017, Vol. 13 Issue 1, p1-14, 14p |
| Abstrakt: |
Introduction: Globally, breast cancer is the most common cancer in women and ranks second most common cause of cancer related mortality. Although efforts are made by researchers in molecular characterization of breast cancer using '-OMIC'S' approaches, limited work has explored to understand the phospholipid alterations in breast cancer. Objectives: This study aims to explore five classes of serum phospholipid alterations in breast cancer towards discrimination of breast cancer from benign and healthy controls. Methods: Twenty eight each of breast cancer patients and age-matched benign and healthy control serum samples were used to identify alterations of phospholipids using liquid chromatography-multiple reaction monitoring-mass spectrometry. Both multivariate and univariate statistical analyses were applied to investigate breast cancer associated phospholipid alterations. Differentially expressed phospholipid species were further confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: Among the identified and quantified 200 phospholipids, 25 phospholipids were found to be statistically significant (VIP > 1.4 and ANOVA p < 0.05) in the serum of women with breast cancer when compared with benign and healthy controls. Comparison of serum phospholipids of breast cancer patients and healthy controls revealed 12 phospholipids were found to be differentially expressed in which six were up-regulated and six were down-regulated. While comparative analysis of breast cancer serum against benign showed an increased concentration of six phospholipids in breast cancer samples. Further, significantly altered phospholipids were structurally characterized by enhanced product ion scanning. Conclusion: Our results demonstrate that some of the differentially regulated phospholipids identified in our study such as PE (14:1/16:0), PC (18:0/18:0), LPE 14:0, PE (20:0/22:2) could be a panel of potential signature which can discriminate breast cancer from benign and healthy controls. These findings also provide insight into lipidomic information that can be used for monitoring of breast cancer progression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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