| Autor: |
Akutsu, N., Milbury, C. M., Burgeson, R. E., Nshiyama, T. |
| Předmět: |
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| Zdroj: |
Experimental Dermatology; Feb99, Vol. 8 Issue 1, p17-21, 5p |
| Abstrakt: |
Type XII and XIV collagens localize near the surface of banded collagen fibrils and most likely work as a molecular bridge between collagen fibrils. We have shown that both collagens can modulate the interact ions between collagen fibrils, allowing fibroblasts to act upon fibrils to Vary the deformability. In the present study the effect of the globular domains (collagenase-resistant domains) of type XII and XIV collagens (XI1-NC-3 and XlV-NC-3) on the migration of fibroblasts into the reconstituted type I collagen gel was investigated. Cell attachment and proliferation on the collagen gel were unaffected. The migration of libroblasts into the gel was increased proportionally to the concentration of collagen. We Found that XlI-NC-3 and XIV-NC-3 domains caused decreases in the numbers of hbrohlasts that migrated into the gel. Heat treatment of XII-NC-3 and XlV-NC-3 or the addition of polyclonal antibodies eliminated the suppressive activity on fibroblast migration. showing that the intact conformation NC-3 domain is important for suppression of migration. The results suggest that both NC'-3 domains influence the deformability of type I collagen fibril networks, which univ cause the change in fibroblast migration. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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