| Autor: |
Mehboob, Farrakh, Oosterkamp, Margreet J., Koehorst, Jasper J., Farrakh, Sumaira, Veuskens, Teun, Plugge, Caroline M., Boeren, Sjef, Vos, Willem M., Schraa, Gosse, Stams, Alfons J.M., Schaap, Peter J. |
| Předmět: |
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| Zdroj: |
Environmental Microbiology; Oct2016, Vol. 18 Issue 10, p3247-3257, 11p |
| Abstrakt: |
Growth of P seudomonas chloritidismutans AW-1 T on C7 to C12 n-alkanes with oxygen or chlorate as electron acceptor was studied by genome and proteome analysis. Whole genome shotgun sequencing resulted in a 5 Mbp assembled sequence with a G + C content of 62.5%. The automatic annotation identified 4767 protein-encoding genes and a putative function could be assigned to almost 80% of the predicted proteins. The distinct phylogenetic position of P . chloritidismutans AW-1 T within the P seudomonas stutzeri cluster became clear by comparison of average nucleotide identity values of sequenced genomes. Analysis of the proteome of P . chloritidismutans AW-1 T showed the versatility of this bacterium to adapt to aerobic and anaerobic growth conditions with acetate or n-decane as substrates. All enzymes involved in the alkane oxidation pathway were identified. An alkane monooxygenase was detected in n-decane-grown cells, but not in acetate-grown cells. The enzyme was found when grown in the presence of oxygen or chlorate, indicating that under both conditions an oxygenase-mediated pathway is employed for alkane degradation. Proteomic and biochemical data also showed that both chlorate reductase and chlorite dismutase are constitutively present, but most abundant under chlorate-reducing conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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