| Autor: |
NOSRATI, Houshang, FEIZI, Mohammad Ali Hosseinpour, LATIFIAN, Farzaneh, HAGHIGHI, Ahmad Razban |
| Předmět: |
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| Zdroj: |
Annals of Oradea University, Biology Fascicle / Analele Universităţii din Oradea, Fascicula Biologie; 2016, Vol. 23 Issue 2, p62-66, 5p |
| Abstrakt: |
Studies on the genetic variation and structure of plant population are important for evaluation of dynamics population and conservation management. We studied the population genetic variation of Onobrychis viciifolia in five ecologically different regions in East-Azerbaijan, Iran using Inter-Simple Sequence Repeats (ISSR) markers. Populations' genetic diversity were estimated based on Nei' and Shannon's indices using polymorphic ISSRs loci. The relationship of populations' geographical and genetic distances and also population altitude and ISSRs variations were investigated. Similarity among populations was assessed by UPGMA clustering analysis. The results showed that polymorphic ISSRs loci varied from 38.75% to 61.25% among populations. Within-population Nei's diversity ranged from 0.1182 to 0.1790. Partitioning of total ISSRs diversity by AMOVA revealed that 67.45% diversity allocated to within- and 32.55% diversity to among-populations, indicating that O. viciifolia is predominantly an outcrossing species. This study showed that population geographical distances was not correlated with Nei distances (N=10, P vale ≥ 0.56) suggesting lack of gene flow among populations. Similarly, the altitude of populations had no impact on populations ISSRs variations (N=5, P value ≥ 0.227). The populations of O. viciifolia growing under salinity stress had both higher and lower ISSRs variability. This cast doubt on Nich-Width Theory, which expects higher variations from stressful populations. Therefore, it can be concluded that populations' genetic patterns in O. viciifolia might be affected by random change, rather than ecological selection. Comparison between the levels of ISSRs variations and those levels of RAPDs previous reported from the same populations showed that RAPDs detect significantly greater variation than ISSRs (N=5, P value ≤0.01, at P ≤ 0.05 level). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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