Ultrasonic synthesis of tyramine derivatives as novel inhibitors of α -glucosidase in vitro.

Autor: Siddiqui, Hina, Bashir, Muhammad Arslan, Javaid, Kulsoom, Nizamani, Arsalan, Bano, Huma, Yousuf, Sammer, Rahman, Atta-ur, Choudhary, M. Iqbal
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Zdroj: Journal of Enzyme Inhibition & Medicinal Chemistry; Dec2016, Vol. 31 Issue 6, p1392-1403, 12p
Abstrakt: Tyramine derivatives3–27were synthesized by using conventional and environmental friendly ultrasonic techniques. These derivatives were then evaluated for the first time for their α-glucosidase (Sources:Saccharomyces cerevisiaeand mammalian rat-intestinal acetone powder) inhibitory activity by usingin vitromechanism-based biochemical assays. Compounds7,14,20,21and26were found to be more active (IC50 = 49.7 ± 0.4, 318.8 ± 3.7, 23.5 ± 0.9, 302.0 ± 7.3 and 230.7 ± 4.0 μM, respectively) than the standard drug, acarbose (IC50 = 840.0 ± 1.73 μM (observed) and 780 ± 0.028 μM (reported)) against α-glucosidase obtained fromSaccharomyces cerevisiae. Kinetic studies were carried out on the most active members of the series in order to determine their mode of inhibition and dissociation constants. Compounds7,20and26were found to be the competitive inhibitors of α-glucosidase. These compounds were also screened for their protein antiglycation, and dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Only compounds20, 22and27showed weak antiglycation activity with IC50values 505.27 ± 5.95, 581.87 ± 5.50 and 440.58 ± 2.74 μM, respectively. All the compounds were found to be inactive against DDP-IV enzyme. Inhibition of α-glucosidase, DPP-IV enzymes and glycation of proteins are valid targets for the discovery of antidiabetic drugs. Cytotoxicity of compounds3–27was also evaluated by using mouse fibroblast 3T3 cell lines. All the compounds were found to be noncytotoxic. The current study describes the synthesis α-glucosidase inhibitory activity of derivatives, based on a natural product tyramine template. The compounds reported here may serve as the starting point for the design and development of novel α-glucosidase inhibitors as antidiabetic agents. [ABSTRACT FROM PUBLISHER]
Databáze: Complementary Index
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