| Autor: |
Wienecke, R., Neubert, U., Volkenandt, M. |
| Předmět: |
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| Zdroj: |
Journal of Cutaneous Pathology; Oct93, Vol. 20 Issue 5, p385-388, 4p |
| Abstrakt: |
A system for the detection of a Borrelia burgdorferi (Bb)-specific gene segment in formalin-fixed, paraffin-embeded skin lesions is described. A nested polymerase chain reaction technique is used to selectively amplify in vitro a short segment of a Bb-specific gene recently described by Rosa et al. (J Infect Dis 1989: 160: 1018). The design of olignonucleotide primers for the amplification of a relatively of DNA which has been altered by fixation in formalin. Using this technique, Bb-specific DNA was clearly identified in 8 of 12 specimens of erthema chronicum migrans and in 1 case of lymphadenosis benigna cutis. These skin lesions are known to represent cutaneous manifestations of Lyme disease. Negative control reactions, using DNA from borrelial strains not related to Lyme disease, were negative. The system enables the dermatopathologist to identify Bb in routinely fixed clinical specimens and allows the rapid analysis of various skin disease for which an association with Bb so far has only been hypothesized. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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