| Autor: |
Madunić, Josip, Matulić, Maja, Friščić, Maja, Pilepić, Kroata Hazler |
| Předmět: |
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| Zdroj: |
Journal of Environmental Science & Health. Part A. Toxic/Hazardous Substances & Environmental Engineering; 2016, Vol. 51 Issue 13, p1157-1163, 7p |
| Abstrakt: |
Cytotoxic activity of 16Hypericumethanolic extracts was evaluated by MTT assay on two human cancer cell lines: glioblastoma A1235 and breast cancer MDA MB-231. Morphology and the type of induced cell death were determined using light and fluorescence microscopy. The majority ofHypericumextracts had no significant cytotoxic effect on MDA MB-231 cells. Eight extracts exhibited mild cytotoxic effect on A1235 cells after 24 h incubation, ranging from 8.0% (H. patulum) to 21.7% (H. oblongifolium). After 72 h of treatment, the strongest inhibition of A1235 viability was observed for extracts ofH. androsaemum(26.4–43.9%),H. balearicum(25.8–36.3%),H. delphicum(14.8–27.4%) andH. densiflorum(11.2–24.1%). Micro-scopic examination of cells showed apoptosis as the dominant type of cell death. Due to observed high viability of treated cells, we propose that cytotoxic effects ofHypericumextracts could be related to alternations/interruptions in the cell cycle. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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