IL-1 β (interleukin-1 β ) stimulates the production and release of multiple cytokines and chemokines by human preadipocytes.

Autor: Alomar, Suliman Y., Gentili, Alessandra, Zaibi, Mohamed S., Kępczyńska, Malgorzata A., Trayhurn, Paul
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Zdroj: Archives of Physiology & Biochemistry; Jul2016, Vol. 122 Issue 3, p117-122, 6p
Abstrakt: The effect of IL-1β on cytokine and chemokine production by human preadipocytes has been examined. Preadipocytes were incubated with IL-1β, and cytokine and chemokine release was measured at 24 h by protein arrays, while the expression of cytokine/chemokine genes was assessed by qPCR at 4 and 24 h. IL-1β stimulated the secretion of multiple cytokines/chemokines, including IL-6, IL-8, IL-10, IL-13, MCP-4, TNFα and IP-10. IL-10 was not released by un-stimulated preadipocytes, while IL-6 exhibited the greatest response to IL-1β (453-fold increase). IL-16 and IL-12p40 did not respond to IL-1β. qPCR demonstrated that IL-1β markedly stimulatedCCL3, CSF3andCXCL10expression at 4 h (>900-fold mRNA increase). A time-course indicated that whileCCL13(encoding MCP-4) exhibited minimal basal expression in preadipocytes, expression increased progressively following differentiation. Human preadipocytes are highly sensitive to IL-1β, the cytokine stimulating a major inflammatory response in these cells similar to that in mature adipocytes. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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