| Autor: |
Visser, Michael, Pieterse, Mervin M., Pinkse, Martijn W. H., Nijsse, Bart, Verhaert, Peter D. E. M., Vos, Willem M., Schaap, Peter J., Stams, Alfons J. M. |
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| Zdroj: |
Environmental Microbiology; Sep2016, Vol. 18 Issue 9, p2843-2855, 13p |
| Abstrakt: |
The S poromusa genus comprises anaerobic spore-forming acetogenic bacteria that stain Gram-negative. S poromusa species typically grow with one-carbon substrates and N-methylated compounds. In the degradation of these compounds methyltransferases are involved. In addition, S poromusa species can grow autotrophically with H2 and CO2, and use a variety of sugars for acetogenic growth. Here we describe a genome analysis of S poromusa strain An4 and a proteome analysis of cells grown under five different conditions. Comparison of the genomes of S poromusa strain An4 and S poromusa ovata strain H1 indicated that An4 is a S . ovata strain. Proteome analysis showed a high abundance of several methyltransferases, predominantly trimethylamine methyltransferases, during growth with betaine, whereas trimethylamine is one of the main end-products of betaine degradation. In methanol degradation methyltransferases are also involved. In methanol-utilizing methanogens, two methyltransferases catalyse methanol conversion, methyltransferase 1 composed of subunits MtaB and MtaC and methyltransferase 2, also called MtaA. The two methyltransferase 1 subunits MtaB and MtaC were highly abundant when strain An4 was grown with methanol. However, instead of MtaA a methyltetrahydrofolate methyltransferase was synthesized. We propose a novel methanol degradation pathway in S poromusa strain An4 that uses a methyltetrahydrofolate methyltransferase instead of MtaA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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