| Autor: |
Friederich, Patrick, Dilger, J. P., Isbrandt, D., Sauter, K., Pongs, O., Urban, B. W. |
| Předmět: |
|
| Zdroj: |
Receptors & Channels; Dec2003, Vol. 9 Issue 6, p387-396, 10p, 3 Diagrams, 2 Charts, 4 Graphs |
| Abstrakt: |
Biophysical properties of delayed rectifier K channels in the human neuroblastoma SH-SY5Y were established using patch clamp recordings. The whole cell K + conductance activated at membrane potentials positive to -20 mV. The midpoint of current activation was 9.6 ± 5.1 mV, the equivalent charge was 3.7 ± .6. Whole-cell currents inactivated slightly with time constants of 700 ms and 5 s. The K + currents were sensitive to micromolar concentrations of TEA and 4-aminopyridine. RT-PCR experiments amplified a cDNA fragment specific for human Kv3.1 channels. Activation gating parameters in outside-out patches were shifted by ∼14 mV in the hyperpolarizing direction. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
